Decationized polyplexes for gene delivery
نویسندگان
چکیده
منابع مشابه
Pectin–Coated Polyethylenimine Polyplexes as Effective Gene Delivery Systems
Gene therapy is believed to be the therapy of the future because it aims to eradicate causes rather than symptoms of diseases. The generic aim of gene therapy is to deliver DNA inside cells in order to achieve a stable and regulated expression of transgenes in the target cells or tissues without side effects. Spontaneous entry of naked DNA inside cells is an inefficient process, consequently, a...
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Local delivery of DNA through a hydrogel scaffold would increase the applicability of gene therapy in tissue regeneration and cancer therapy. However, the delivery of DNA/cationic polymer nanoparticles (polyplexes) using hydrogels is challenging due to the aggregation and inactivation of polyplexes during their incorporation into hydrogel scaffolds. We developed a novel process (termed caged na...
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Various cationic polypeptides of linear or highly branched structures were synthesized by introducing tertiary or quaternary ammonium groups and hydroxyl groups to poly(L-lysine) (PL) or polyamideamine (PAMAM) dendrimers. These polycations were mixed with plasmid DNA to form polyplexes and subjected to in vitro gene introduction experiment. The transient gene expression was greatly affected by ...
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Small interfering RNA (siRNA) targeted therapeutics (STT) offers a compelling alternative to tradition medications for treatment of genetic diseases by providing a means to silence the expression of specific aberrant proteins, through interference at the expression level. The perceived advantage of siRNA therapy is its ability to target, through synthetic antisense oligonucleotides, any part of...
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ژورنال
عنوان ژورنال: Expert Opinion on Drug Delivery
سال: 2014
ISSN: 1742-5247,1744-7593
DOI: 10.1517/17425247.2015.988136